in vitro Receptor Autoradiography

With the use of radiolabeled ligands, in vitro Autoradiography studies can be used to localize receptors (receptor distribution) and characterize drug binding sites in tissue sections.

For these studies, cryostat-cut sections are incubated in vitro in a receptor specific buffer containing the radiolabeled drug. Once equilibrium binding conditions are achieved, the sections are washed, dried and imaged for radioactivity using either phosphor-imaging or beta imaging.