Dilution refers to make a lower concentration solution from higher concentrations. Solutions usually are stored in a higher concentration, for convenience of use and avoiding contamination.

The METIS Labs dilution calculator is a useful tool which allows you to calculate the:

  1. prepare stock solutions
  2. dilutions from stock solutions
  3. serial dilutions of stock solutions

Calculate the dilution required to prepare a stock solution

Calc

The dilution calculator is based on the following equation:

Concentration(start) x Volume(start) = Concentration(final) x Volume(final)

An example of a dilution calculation using the dilution calculator
What volume of a given 10 mM stock solution is required to make 20  ml of a 50 μM solution?
Using the equation C1 V1 = C2 V2, where C1=10 mM, C2=50 μM, V2=20 ml and V1 is the unknown:

  1. Enter 10 into the Concentration (start) box and select the correct unit (millimolar)
  2. Enter 50 into the Concentration (final) box and select the correct unit (micromolar)
  3. Enter 20 into the Volume (final) box and select the correct unit (milliliter)
  4. Press calculate
  5. The answer of 100 microliter (0.1 ml) appears in the Volume (start) box