Dilution refers to make a lower concentration solution from higher concentrations. Solutions usually are stored in a higher concentration, for convenience of use and avoiding contamination.
The METIS Labs dilution calculator is a useful tool which allows you to calculate the:
- prepare stock solutions
- dilutions from stock solutions
- serial dilutions of stock solutions
Calculate the dilution required to prepare a stock solution
The dilution calculator is based on the following equation:
Concentration(start) x Volume(start) = Concentration(final) x Volume(final)
An example of a dilution calculation using the dilution calculator
What volume of a given 10 mM stock solution is required to make 20 ml of a 50 μM solution?
Using the equation C1 V1 = C2 V2, where C1=10 mM, C2=50 μM, V2=20 ml and V1 is the unknown:
- Enter 10 into the Concentration (start) box and select the correct unit (millimolar)
- Enter 50 into the Concentration (final) box and select the correct unit (micromolar)
- Enter 20 into the Volume (final) box and select the correct unit (milliliter)
- Press calculate
- The answer of 100 microliter (0.1 ml) appears in the Volume (start) box