Profiling Services

Proteomic Profiling   

METIS Labs can radiolabel your small molecule, antibody, or protein to screen for interactions with thousands of human proteins immobilized on microarray chips.  Profiling like this aids with drug target discovery and with target validation (confirm specificity, identify crossreactions). Two platforms are available: Invitrogen’s ProtoArray® and CDI’s HuProt™ array.

Proteomic microarry chip layout and example
Click to enlarge
HuProt and ProtoArray categories

Radiolabeled samples interacting with immobilized proteins produce microscopic spots of varying intensity that we can detect with our digital imaging equipment.  The resulting images are processed and statistically analyzed with GenePix®-based software.  METIS Labs then produces a detailed report of the profiling experiment and results for the client.

METIS offers a choice of microarrays that allow clients to screen ~45% to ~85% of the entire human proteome in a high throughput methodology.  Invitrogen’s ProtoArray® is the most well known and widely used proteomic microarray.  CDI’s HuProt™ array is a newer and significantly larger array.  About 4,900 proteins are exactly the same on both products; the rest are either variants of the same gene/protein family or uniquely different.

HuProt proteomic profiling microarray chips

Ordering Information   

Speak with one of our scientists so that the right project can be designed for you.  A new project will require a minimum of 4 sets of chips (1 positive control, 3 test conditions); typically a project will include additional chips for an unlabeled “cold” competitor and/or replicates.  Telephone us at +1.888.295.7550 or click on the request quote button   

Invitrogen™ and ProtoArray® are registered trademarks of Thermo Fisher Scientific Inc.; HuProt™ is a registered trademark of CDI Laboratories Inc.  These are used with permission for informational purposes only and no endorsement by these companies is implied.